edward boyden (Addgene inc)
Addgene inc is a verified supplier 
96
Structured Review
Addgene inc
edward boyden
Edward Boyden, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/edward+boyden/pAAV-CAG-tdTomato+(codon+diversified)+(Plasmid+%2359462)/pmc13000482-297-31-33
Average 96 stars, based on 277 article reviews
Edward Boyden, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/edward+boyden/pAAV-CAG-tdTomato+(codon+diversified)+(Plasmid+%2359462)/pmc13000482-297-31-33
Average 96 stars, based on 277 article reviews
edward boyden - by Bioz Stars,
2026-09
96/100 stars
Images
Related Articles
Plasmid Preparation:Article Title: Evaluation of Lipid Nanoparticles as Vehicles for Optogenetic Delivery in Primary Cortical Neurons. Article Snippet: To achieve the desired concentration of LNP formulations, samples were subjected to centrifugation using a 10K MWCO Amicon® Ultra Centrifugal filter (Merck KGaA, Darmstadt, Germany) at 2000× g and 4 ◦C until the target volume was obtained. .. The pChrimsonR plasmid embedded into the LNPs was obtained from Article Title: Evaluation of Lipid Nanoparticles as Vehicles for Optogenetic Delivery in Primary Cortical Neurons Article Snippet: To achieve the desired concentration of LNP formulations, samples were subjected to centrifugation using a 10K MWCO Amicon ® Ultra Centrifugal filter (Merck KGaA, Darmstadt, Germany) at 2000× g and 4 °C until the target volume was obtained. .. The pChrimsonR plasmid embedded into the LNPs was obtained from Article Title: Hyperinnervation inhibits organ-level regeneration in mammalian skin. Article Snippet: All mice were maintained in an AAALAC-accredited (Association for Assessment and Accreditation of Laboratory Animal Care) animal facility at Harvard University. .. pAAV-CAG-tdTomato was a gift from Article Title: A developmental switch in corticoclaustral signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Article Title: Virus-mediated gene transfer of soluble amyloid precursor protein-alpha via systemic injection in a mouse model of Alzheimer's disease. Article Snippet: The mouse colony was obtained by donation from the Mutant Mouse Resource Research Center. .. The pAAV-CAG-tdTomato (codon diversified) was a gift from Amplification:Article Title: Evaluation of Lipid Nanoparticles as Vehicles for Optogenetic Delivery in Primary Cortical Neurons. Article Snippet: To achieve the desired concentration of LNP formulations, samples were subjected to centrifugation using a 10K MWCO Amicon® Ultra Centrifugal filter (Merck KGaA, Darmstadt, Germany) at 2000× g and 4 ◦C until the target volume was obtained. .. The pChrimsonR plasmid embedded into the LNPs was obtained from Article Title: Evaluation of Lipid Nanoparticles as Vehicles for Optogenetic Delivery in Primary Cortical Neurons Article Snippet: To achieve the desired concentration of LNP formulations, samples were subjected to centrifugation using a 10K MWCO Amicon ® Ultra Centrifugal filter (Merck KGaA, Darmstadt, Germany) at 2000× g and 4 °C until the target volume was obtained. .. The pChrimsonR plasmid embedded into the LNPs was obtained from Purification:Article Title: Evaluation of Lipid Nanoparticles as Vehicles for Optogenetic Delivery in Primary Cortical Neurons. Article Snippet: To achieve the desired concentration of LNP formulations, samples were subjected to centrifugation using a 10K MWCO Amicon® Ultra Centrifugal filter (Merck KGaA, Darmstadt, Germany) at 2000× g and 4 ◦C until the target volume was obtained. .. The pChrimsonR plasmid embedded into the LNPs was obtained from Article Title: Evaluation of Lipid Nanoparticles as Vehicles for Optogenetic Delivery in Primary Cortical Neurons Article Snippet: To achieve the desired concentration of LNP formulations, samples were subjected to centrifugation using a 10K MWCO Amicon ® Ultra Centrifugal filter (Merck KGaA, Darmstadt, Germany) at 2000× g and 4 °C until the target volume was obtained. .. The pChrimsonR plasmid embedded into the LNPs was obtained from Control:Article Title: Hyperinnervation inhibits organ-level regeneration in mammalian skin. Article Snippet: All mice were maintained in an AAALAC-accredited (Association for Assessment and Accreditation of Laboratory Animal Care) animal facility at Harvard University. .. pAAV-CAG-tdTomato was a gift from Bioprocessing:Article Title: Hyperinnervation inhibits organ-level regeneration in mammalian skin. Article Snippet: All mice were maintained in an AAALAC-accredited (Association for Assessment and Accreditation of Laboratory Animal Care) animal facility at Harvard University. .. pAAV-CAG-tdTomato was a gift from Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Injection:Article Title: A developmental switch in corticoclaustral signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Virus:Article Title: Environmental enrichment preserves retrosplenial parvalbumin density and cognitive function in female 5xFAD mice Article Snippet: .. The pAAV-FLEX-tdTomato virus used in these experiments 22 JN eu ros ci A cce pte d M an us rip t 2 was also a gift from Recombinant:Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Mutagenesis:Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from Construct:Article Title: Targeting of Itch by clomipramine or gene therapy improves cognitive defects related to Alzheimer’s disease Article Snippet: .. For preparation of recombinant AAV, mouse WT or T222A/S232A or K393R mutant Itch was subcloned from the constructs described previously in pAAV-CAG-tdTomato vector [(pAAV-CAG-tdTomato (codon diversified)], which was a gift from |